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J Transl Med. 2015 Jul 14;13:223. doi: 10.1186/s12967-015-0576-z.

Metabolic signatures differentiate ovarian from colon cancer cell lines.

Author information

  • 1Department of Physiology and Biophysics, Weill Cornell Medical College in Qatar, Qatar-Foundation, P.O. Box 24144, Doha, Qatar. amh2025@qatar-med.cornell.edu.
  • 2Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar. bguerrouahen@sidra.org.
  • 3Department of Genetic Medicine, Weill Cornell Medical College, New York, NY, 10065, USA. bguerrouahen@sidra.org.
  • 4Experimental Biology Division-Research, Sidra Medical and Research Center, PO Box 26999, Doha, Qatar. bguerrouahen@sidra.org.
  • 5Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar. jep2026@qatar-med.cornell.edu.
  • 6Department of Genetic Medicine, Weill Cornell Medical College, New York, NY, 10065, USA. jep2026@qatar-med.cornell.edu.
  • 7Department of Physiology and Biophysics, Weill Cornell Medical College in Qatar, Qatar-Foundation, P.O. Box 24144, Doha, Qatar. ild2003@qatar-med.cornell.edu.
  • 8Metabolon, Inc., Durham, NC, 27713, USA. EKaroly@metabolon.com.
  • 9Department of Physiology and Biophysics, Weill Cornell Medical College in Qatar, Qatar-Foundation, P.O. Box 24144, Doha, Qatar. kas2049@qatar-med.cornell.edu.
  • 10Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany. kas2049@qatar-med.cornell.edu.
  • 11Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar. jat2021@qatar-med.cornell.edu.
  • 12Department of Genetic Medicine, Weill Cornell Medical College, New York, NY, 10065, USA. jat2021@qatar-med.cornell.edu.
  • 13Department of Genetic Medicine and Obstetrics and Gynecology, Weill Cornell Medical College, Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Qatar-Foundation, P.O. Box 24144, Doha, Qatar. jat2021@qatar-med.cornell.edu.

Abstract

BACKGROUND:

In this era of precision medicine, the deep and comprehensive characterization of tumor phenotypes will lead to therapeutic strategies beyond classical factors such as primary sites or anatomical staging. Recently, "-omics" approached have enlightened our knowledge of tumor biology. Such approaches have been extensively implemented in order to provide biomarkers for monitoring of the disease as well as to improve readouts of therapeutic impact. The application of metabolomics to the study of cancer is especially beneficial, since it reflects the biochemical consequences of many cancer type-specific pathophysiological processes. Here, we characterize metabolic profiles of colon and ovarian cancer cell lines to provide broader insight into differentiating metabolic processes for prospective drug development and clinical screening.

METHODS:

We applied non-targeted metabolomics-based mass spectroscopy combined with ultrahigh-performance liquid chromatography and gas chromatography for the metabolic phenotyping of four cancer cell lines: two from colon cancer (HCT15, HCT116) and two from ovarian cancer (OVCAR3, SKOV3). We used the MetaP server for statistical data analysis.

RESULTS:

A total of 225 metabolites were detected in all four cell lines; 67 of these molecules significantly discriminated colon cancer from ovarian cancer cells. Metabolic signatures revealed in our study suggest elevated tricarboxylic acid cycle and lipid metabolism in ovarian cancer cell lines, as well as increased β-oxidation and urea cycle metabolism in colon cancer cell lines.

CONCLUSIONS:

Our study provides a panel of distinct metabolic fingerprints between colon and ovarian cancer cell lines. These may serve as potential drug targets, and now can be evaluated further in primary cells, biofluids, and tissue samples for biomarker purposes.

PMID:
26169745
PMCID:
PMC4499939
DOI:
10.1186/s12967-015-0576-z
[PubMed - indexed for MEDLINE]
Free PMC Article
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