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Biochem Biophys Res Commun. 2015 Aug 28;464(3):800-6. doi: 10.1016/j.bbrc.2015.07.038. Epub 2015 Jul 10.

Anti-infective peptide IDR-1002 augments monocyte chemotaxis towards CCR5 chemokines.

Author information

1
Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, 2259 Lower Mall Research Station, University of British Columbia, Vancouver, V6T 1Z4, Canada.
2
Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, 2259 Lower Mall Research Station, University of British Columbia, Vancouver, V6T 1Z4, Canada. Electronic address: bob@hancocklab.com.

Abstract

Innate defense regulator (IDR) peptides are a class of immunomodulators which enhance and modulate host innate immune responses against microbial pathogens. While IDR-mediated protection against a range of bacterial pathogens is dependent on enhanced monocyte recruitment to the site of infection, the mechanisms through which they increase monocyte trafficking remain unclear. In this study, anti-infective peptide IDR-1002 was shown to enhance monocyte chemotaxis towards chemokines CCL3 and CCL5. This enhancement correlated with the selective upregulation of CCR5 surface expression by peptide-treated monocytes. It was found that IDR-1002 enhancement of monocyte chemotaxis was fully dependent on CCR5 function. Furthermore, IDR-1002 enhanced chemokine-induced monocyte p38 MAPK phosphorylation in a CCR5-dependent fashion. Overall, these results indicate that peptide IDR-1002 can selectively influence monocyte recruitment by host chemokines through the regulation of chemokine receptors.

KEYWORDS:

Chemokine receptors; Chemokines; Immunomodulation; Innate defense regulators; Monocytes

PMID:
26168734
DOI:
10.1016/j.bbrc.2015.07.038
[Indexed for MEDLINE]

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