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Anticancer Res. 2015 Aug;35(8):4487-92.

Degalactosylated/Desialylated Bovine Colostrum Induces Macrophage Phagocytic Activity Independently of Inflammatory Cytokine Production.

Author information

1
Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan uto.yoshihiro@tokushima-u.ac.jp.
2
Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan.
3
Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan Saisei Mirai Cell Processing Center, Osaka, Japan.
4
Saisei Mirai Cell Processing Center, Osaka, Japan.
5
Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan Saisei Mirai Cell Processing Center, Osaka, Japan Kobe Saisei Mirai Clinic, Kobe, Japan Inui Immunotherapy Clinic, Osaka, Japan.
6
Inui Immunotherapy Clinic, Osaka, Japan.
7
Kohkan Pharmaceutical Institute Co., Ltd., Tokyo, Japan.
8
Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Tottori, Japan.

Abstract

BACKGROUND/AIM:

Colostrum contains antibodies, such as immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM), and, therefore, has potent immunomodulating activity. In particular, IgA has an O-linked sugar chain similar to that in the group-specific component (Gc) protein, a precursor of the Gc protein-derived macrophage-activating factor (GcMAF). In the present study, we investigated the macrophage-activating effects of degalactosylated/desialylated bovine colostrum.

RESULTS:

We detected the positive band in degalactosylated/ desialylated bovine colostrum by western blotting using Helix pomatia agglutinin lectin. We also found that degalactosylated/ desialylated bovine colostrum could significantly enhance the phagocytic activity of mouse peritoneal macrophages in vitro and of intestinal macrophages in vivo. Besides, degalactosylated/desialylated bovine colostrum did not mediate the production of inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β).

CONCLUSION:

Similar to the use of GcMAF, degalactosylated/desialylated bovine colostrum can be used as a potential macrophage activator for various immunotherapies.

KEYWORDS:

GcMAF; degalactosylated/desialylated bovine colostrum; macrophage phagocytic activity; mouse intestinal macrophage; mouse peritoneal macrophage

PMID:
26168491
[Indexed for MEDLINE]

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