Format

Send to

Choose Destination
Anticancer Res. 2015 Aug;35(8):4473-7.

Molecular Response of Human Monocytes Following Interaction with Colon Cancer Cells by Pre-treatment with Low-dose Lipopolysaccharide.

Author information

1
Department of Medical Technology, School of Life and Environmental Science, Azabu University, Kanagawa, Japan hondat@azabu-u.ac.jp.
2
Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University, Kagawa, Japan.

Abstract

BACKGROUND/AIM:

The increased mRNA expression of chemotaxis- and angiogenesis-related factors in human monocytes following interaction with colon cancer cells has been shown to be suppressed by pre-treatment with low-dose lipopolysaccharide (LPS) (100 pg/ml). It has been demonstrated that low-dose LPS reduced the expression of RelB, a member of the nuclear factor (NF)-κB transcription factor family, in mouse macrophages and the NF-κB signaling pathway was important for tumor initiation and growth in tumor-associated macrophages. In addition, the signal transducer and activator of transcription 3 (STAT3) regulated innate immunity via Toll-like receptor (TLR)4 signaling. In the present study, the mRNA expression of signaling pathway- and suppression-related genes in human monocytes following a low-dose LPS treatment and subsequent interaction with colon cancer cells was investigated, in order to assess the molecular response.

MATERIALS AND METHODS:

The human monocyte cell line THP-1 was treated with LPS and, subsequently, co-cultured with the human colon cancer cell line DLD-1. The mRNA expression of various genes was then analyzed using quantitative real-time polymerase chain reaction (PCR).

RESULTS:

The mRNA expression of RelB, STAT3, interleukin (IL)-10 and transforming growth factor (TGF)-β in THP-1 cells following interaction with DLD-1 cells was suppressed by pre-treatment with low-dose LPS (100 pg/ml).

CONCLUSION:

Treating human monocytes with low-dose LPS may be useful for suppressing tumor progression and may be valuable for maintaining homeostasis.

KEYWORDS:

RelB; STAT3; co-culture; colon cancer cell; low-dose lipopolysaccharide; monocyte

PMID:
26168489
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center