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Neurosci Res. 2015 Nov;100:55-62. doi: 10.1016/j.neures.2015.06.006. Epub 2015 Jul 10.

Low-cost three-dimensional gait analysis system for mice with an infrared depth sensor.

Author information

1
Graduate School of Information Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan. Electronic address: nakamura.akihiro.mt5@is.naist.jp.
2
Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan. Electronic address: funaya@brain.kyutech.ac.jp.
3
Department of Stem Cell Biology and Medicine, Kyushu University, Fukuoka, Japan. Electronic address: uezono@scb.med.kyushu-u.ac.jp.
4
Department of Stem Cell Biology and Medicine, Kyushu University, Fukuoka, Japan. Electronic address: kin1@scb.med.kyushu-u.ac.jp.
5
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan. Electronic address: ishiday@bs.naist.jp.
6
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center, Ibaraki, Japan. Electronic address: suzukito@brc.riken.jp.
7
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center, Ibaraki, Japan. Electronic address: swakana@brc.riken.jp.
8
Graduate School of Information Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan; Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan. Electronic address: tom@brain.kyutech.ac.jp.

Abstract

Three-dimensional (3D) open-field gait analysis of mice is an essential procedure in genetic and nerve regeneration research. Existing gait analysis systems are generally expensive and may interfere with the natural behaviors of mice because of optical markers and transparent floors. In contrast, the proposed system captures the subjects shape from beneath using a low-cost infrared depth sensor (Microsoft Kinect) and an opaque infrared pass filter. This means that we can track footprints and 3D paw-tip positions without optical markers or a transparent floor, thereby preventing any behavioral changes. Our experimental results suggest with healthy mice that they are more active on opaque floors and spend more time in the center of the open-field, when compared with transparent floors. The proposed system detected footprints with a comparable performance to existing systems, and precisely tracked the 3D paw-tip positions in the depth image coordinates.

KEYWORDS:

Depth image; Locomotion; Open-field test; Pose reconstruction; Rodents; Three-dimensional gait analysis

PMID:
26166585
DOI:
10.1016/j.neures.2015.06.006
[Indexed for MEDLINE]
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