Format

Send to

Choose Destination
Neurosci Lett. 2015 Aug 18;602:145-9. doi: 10.1016/j.neulet.2015.07.005. Epub 2015 Jul 7.

Alpha-synuclein immunoreactivity patterns in the enteric nervous system.

Author information

1
Department of Pathology, Barcelona, Spain.
2
Parkinson's Disease and Movement Disorders Unit Neurology Service Institut de Neurociències Hospital Clínic, University of Barcelona, Barcelona, Spain; Institut d'Investigacions Biomèdiques August Pi I Sunyer-IDIBAPS, Barcelona, Spain.
3
Department of Neurology, Medical University of Innsbruck, Austria.
4
Department of Pathology, Barcelona, Spain; Tumour Bank of the Biobank, Hospital Clinic, IDIBAPS, Barcelona, Spain.
5
Neurological Tissue Bank of the Biobank, Hospital Clinic, IDIBAPS, Barcelona, Spain.
6
Parkinson's Disease and Movement Disorders Unit Neurology Service Institut de Neurociències Hospital Clínic, University of Barcelona, Barcelona, Spain; Institut d'Investigacions Biomèdiques August Pi I Sunyer-IDIBAPS, Barcelona, Spain; Centro en Red para la Investigación de Enfermedades Neurodegenerativas CIBERNED, Spain. Electronic address: etolosa@clinic.ub.es.

Abstract

We aimed to compare immunoreactivity patterns of four different anti-α-syn antibodies in surgical specimens of the gastrointestinal tract of Parkinson disease and control cases. Surgical specimens from stomach, small and large bowel of 6 PD cases and 12 controls were studied. Primary antibodies: anti-α-syn clone KM51, anti-phosphorylated α-syn Ser129, anti-α-syn clone 15G7 and anti-nitrated α-syn505. We found different immunoreactivity patterns: (a) coarse, Lewy-body-like aggregates labelled by the 4 antibodies and detected in 4/6 PD cases and in 1/12 controls; (b) distinct punctate cytoplasmic staining of ganglion cells labelled by anti-phosphorylated-α-syn and detected in 3/6 PD cases and 3/12 controls; (c) fine diffuse, synaptic-type staining of neural structures labelled by anti-α-syn-15G7 and anti-nitrated-α-syn505 and detected in all subjects. We conclude that different specific and non-specific immunoreactivity patterns are detected in surgical specimens of gastrointestinal tract when using different anti-α-syn antibodies, as they recognize different epitopes and states of alpha-synuclein protein. Coarse aggregates in neural structures seem to be the most promising marker for the diagnosis of Lewy-body parkinsonism when evaluating abnormal α-syn in the gastrointestinal tract.

KEYWORDS:

Alpha-synuclein; Enteric nervous system; Gastrointestinal tract; Lewy pathology; Parkinson disease

PMID:
26163460
DOI:
10.1016/j.neulet.2015.07.005
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center