Format

Send to

Choose Destination
Vet Res. 2015 Jul 11;46:80. doi: 10.1186/s13567-015-0212-1.

Extracellular cyclophilin A possesses chemotaxic activity in cattle.

Author information

1
Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. satoru.takanashi.p2@dc.tohoku.ac.jp.
2
International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. satoru.takanashi.p2@dc.tohoku.ac.jp.
3
Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. nochi@m.tohoku.ac.jp.
4
International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. nochi@m.tohoku.ac.jp.
5
Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. miku.abe.s2@dc.tohoku.ac.jp.
6
International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. miku.abe.s2@dc.tohoku.ac.jp.
7
Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. vo1pe_d1_frago1a-02@docomo.ne.jp.
8
Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. urakawa@bios.tohoku.ac.jp.
9
International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. urakawa@bios.tohoku.ac.jp.
10
Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. katsuyoshi-sato@akita-pu.ac.jp.
11
Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. zhuang.tao.r3@dc.tohoku.ac.jp.
12
International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. zhuang.tao.r3@dc.tohoku.ac.jp.
13
Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. gmag.529@gmail.com.
14
National Agriculture and Food Research Organization, National Institute of Animal Health, Hokkaido, 062-0045, Japan. hayatomo@affrc.go.jp.
15
National Agriculture and Food Research Organization, National Institute of Animal Health, Hokkaido, 062-0045, Japan. yokiku@affrc.go.jp.
16
International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. haruki@bios.tohoku.ac.jp.
17
Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. haruki@bios.tohoku.ac.jp.
18
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Cardiganshire, SY23 3DA, UK. mir@aber.ac.uk.
19
Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. watakoh@bios.tohoku.ac.jp.
20
International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. watakoh@bios.tohoku.ac.jp.
21
Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. asosan@bios.tohoku.ac.jp.
22
International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. asosan@bios.tohoku.ac.jp.

Abstract

Cyclophilin A (CyPA) was originally discovered in bovine thymocytes as a cytosolic binding protein of the immunosuppressive drug cyclosporine A. Recent studies have revealed that in mice and humans, CyPA is secreted from cells in injured or infected tissues and plays a role in recruiting inflammatory cells in those tissues. Here we found that in cattle abundant level of extracellular CyPA was observed in tissues with inflammation. To aid in investigating the role of extracellular CyPA in cattle, we generated recombinant bovine CyPA (rbCyPA) and tested its biological activity as an inflammatory mediator. When bovine peripheral blood cells were treated with rbCyPA in vitro, we observed that rbCyPA reacts with the membranous surface of granulocytes, monocytes and lymphocytes. Chemotaxis analysis showed that the granulocytes migrate toward rbCyPA and the migration is inhibited by pre-treatment with an anti-bovine CyPA antibody. These results indicate that, as for mice and humans, extracellular CyPA possesses chemotactic activity to recruit inflammatory cells (e.g., granulocytes) in cattle, and could thus be a potential therapeutic target for the treatment of inflammation.

PMID:
26163364
PMCID:
PMC4498507
DOI:
10.1186/s13567-015-0212-1
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center