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J Leukoc Biol. 2015 Sep;98(3):319-32. doi: 10.1189/jlb.4RI0115-006RR. Epub 2015 Jul 10.

Elucidation of monocyte/macrophage dynamics and function by intravital imaging.

Author information

1
Viral Immunology and Intravital Imaging Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA.
2
Viral Immunology and Intravital Imaging Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA mcgavernd@mail.nih.gov.

Abstract

Monocytes and macrophages are a diverse population of innate immune cells that play a critical role in homeostasis and inflammation. These cells are surveillant by nature and closely monitor the vasculature and surrounding tissue during states of health and disease. Given their abundance and strategic positioning throughout the body, myeloid cells are among the first responders to any inflammatory challenge and are active participants in most immune-mediated diseases. Recent studies have shed new light on myeloid cell dynamics and function by use of an imaging technique referred to as intravital microscopy (IVM). This powerful approach allows researchers to gain real-time insights into monocytes and macrophages performing homeostatic and inflammatory tasks in living tissues. In this review, we will present a contemporary synopsis of how intravital microscopy has revolutionized our understanding of myeloid cell contributions to vascular maintenance, microbial defense, autoimmunity, tumorigenesis, and acute/chronic inflammatory diseases.

KEYWORDS:

infection; microscopy; myeloid cells; tumors; two-photon; vasculature

PMID:
26162402
PMCID:
PMC4763596
DOI:
10.1189/jlb.4RI0115-006RR
[Indexed for MEDLINE]
Free PMC Article

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