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RNA Biol. 2015;12(8):893-9. doi: 10.1080/15476286.2015.1063773.

MYCNOS functions as an antisense RNA regulating MYCN.

Author information

1
a Molecular and Experimental Medicine ; The Scripps Research Institute ; La Jolla , CA USA.

Abstract

Amplification or overexpression of neuronal MYC (MYCN) is associated with poor prognosis of human neuroblastoma. Three isoforms of the MYCN protein have been described as well as a protein encoded by an antisense transcript (MYCNOS) that originates from the opposite strand at the MYCN locus. Recent findings suggest that some antisense long non-coding RNAs (lncRNAs) can play a role in epigenetically regulating gene expression. Here we report that MYCNOS transcripts function as a modulator of the MYCN locus, affecting MYCN promoter usage and recruiting various proteins, including the Ras GTPase-activating protein-binding protein G3BP1, to the upstream MYCN promoter. Overexpression of MYCNOS results in a reduction of upstream MYCN promoter usage and increased MYCN expression, suggesting that the protein-coding MYCNOS also functions as a regulator of MYCN ultimately controlling MYCN transcriptional variants. The observations presented here demonstrate that protein-coding transcripts can regulate gene transcription and can tether regulatory proteins to target loci.

KEYWORDS:

G3BP1; MAP4; RNA; Transcriptional regulation; bidirectional promoter; neuroblastoma

PMID:
26156430
PMCID:
PMC4615167
DOI:
10.1080/15476286.2015.1063773
[Indexed for MEDLINE]
Free PMC Article

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