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Plant Cell Rep. 2015 Oct;34(10):1849-56. doi: 10.1007/s00299-015-1832-7. Epub 2015 Jul 9.

Steroid-inducible BABY BOOM system for development of fertile Arabidopsis thaliana plants after prolonged tissue culture.

Author information

1
Farmingdale State College, Hale Hall, 2350 Broadhollow Road, Farmingdale, NY, 11735, USA. lutzk@farmingdale.edu.
2
Farmingdale State College, Hale Hall, 2350 Broadhollow Road, Farmingdale, NY, 11735, USA.
3
Rutgers The State University of NJ, Waksman Institute of Microbiology, 190 Frelinghuysen Road, Piscataway, NJ, 08854, USA.

Abstract

We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana regeneration using a regulated system to induce embryogenic cultures from leaf tissue. The system is based on BABY BOOM (BBM), a transcription factor that turns on genes involved in embryogenesis. We transformed the nucleus of A. thaliana plants with BBM:GR, a gene in which the BBM coding region is fused with the glucocorticoid receptor (GR) steroid-binding domain. In the absence of the synthetic steroid dexamethasone (DEX), the BBM:GR fusion protein is localized in the cytoplasm. Only when DEX is included in the culture medium does the BBM transcription factor enter the nucleus and turn on genes involved in embryogenesis. BBM:GR plant lines show prolific shoot regeneration from leaf pieces on media containing DEX. Removal of DEX from the culture media allowed for flowering and seed formation. Therefore, use of BBM:GR leaf tissue for regeneration of plants for extended periods of time in tissue culture will facilitate the recovery of fertile plants.

KEYWORDS:

Arabidopsis thaliana; BABY BOOM; Plant regeneration; Tissue culture

PMID:
26156330
DOI:
10.1007/s00299-015-1832-7
[Indexed for MEDLINE]

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