Format

Send to

Choose Destination
See comment in PubMed Commons below
Cell Metab. 2015 Jul 7;22(1):100-12. doi: 10.1016/j.cmet.2015.06.001.

The Gut Microbiota Regulates Intestinal CD4 T Cells Expressing RORγt and Controls Metabolic Disease.

Author information

1
Institut des Maladies Métaboliques et Cardiovasculaires, INSERM U1048 F-31432 Toulouse, France; Université Paul Sabatier, F-31432 Toulouse, France. Electronic address: lucile.garidou@gmail.com.
2
Institut des Maladies Métaboliques et Cardiovasculaires, INSERM U1048 F-31432 Toulouse, France; Université Paul Sabatier, F-31432 Toulouse, France.
3
Université Paul Sabatier, F-31432 Toulouse, France; Centre de Physiopathologie de Toulouse Purpan, INSERM U1043, F-31300 Toulouse, France; CNRS, UMR5282, F-31300 Toulouse, France.
4
Danisco Sweeteners Oy Sokeritehtaantie 20 FI-02460 Kantvik, Finland.
5
Plateau de Bioinformatique et Biostatistique, INSERM UMR1048, F-31432 Toulouse, France.
6
Vaiomer SAS, 516 Rue Pierre et Marie Curie, F-31670 Labège, France.
7
Université Paul Sabatier, F-31432 Toulouse, France; Hôpital Rangueil, Département Thérapeutique, F-31059 Toulouse, France.
8
Institut Pasteur, Unité de Développement des Tissus Lymphoïdes, F-75724 Paris, France.
9
Institut des Maladies Métaboliques et Cardiovasculaires, INSERM U1048 F-31432 Toulouse, France; Université Paul Sabatier, F-31432 Toulouse, France. Electronic address: remy.burcelin@inserm.fr.

Abstract

A high-fat diet (HFD) induces metabolic disease and low-grade metabolic inflammation in response to changes in the intestinal microbiota through as-yet-unknown mechanisms. Here, we show that a HFD-derived ileum microbiota is responsible for a decrease in Th17 cells of the lamina propria in axenic colonized mice. The HFD also changed the expression profiles of intestinal antigen-presenting cells and their ability to generate Th17 cells in vitro. Consistent with these data, the metabolic phenotype was mimicked in RORγt-deficient mice, which lack IL17 and IL22 function, and in the adoptive transfer experiment of T cells from RORγt-deficient mice into Rag1-deficient mice. We conclude that the microbiota of the ileum regulates Th17 cell homeostasis in the small intestine and determines the outcome of metabolic disease.

PMID:
26154056
DOI:
10.1016/j.cmet.2015.06.001
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center