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Bioelectrochemistry. 2015 Dec;106(Pt B):335-42. doi: 10.1016/j.bioelechem.2015.06.009. Epub 2015 Jun 30.

Scaffold electrodes based on thioctic acid-capped gold nanoparticles coordinated Alcohol Dehydrogenase and Azure A films for high performance biosensor.

Author information

1
Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049, Spain. Electronic address: cesar.anquela@uam.es.
2
Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049, Spain. Electronic address: tania.garcia@uam.es.
3
Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049, Spain. Electronic address: josemaria.abad@uam.es.
4
Instituto de Catalisis y Petroleoquimica, CSIC. C/ Marie Curie, 2, L10., 28049 Madrid, Spain. Electronic address: marcospita@icp.csic.es.
5
Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049, Spain. Electronic address: felix.pariente@uam.es.
6
Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049, Spain; IMDEA Nanociencia, Madrid, Spain. Electronic address: encarnacion.lorenzo@uam.es.

Abstract

Nanometric size gold nanoparticles capped with thiotic acid are used to coordinate with the Zn (II) present in the catalytic center of Alcohol Dehydrogenase (ADH). In combination with the NADH oxidation molecular catalyst Azure A, electrografted onto carbon screen-printed electrodes, they are used as scaffold electrodes for the construction of a very efficient ethanol biosensor. The final biosensing device exhibits a highly efficient ethanol oxidation with low overpotential of -0.25 V besides a very good analytical performance with a detection limit of 0.14±0.01 μM and a stable response for more than one month.

KEYWORDS:

Alcohol dehydrogenase; Biosensor; Electrografting; Gold nanoparticles

[Indexed for MEDLINE]

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