Format

Send to

Choose Destination
J Microbiol Methods. 2015 Oct;117:11-3. doi: 10.1016/j.mimet.2015.07.001. Epub 2015 Jul 4.

Development of an SNP-based PCR assay for rapid differentiation of a Japanese live vaccine strain from field isolates of Erysipelothrix rhusiopathiae.

Author information

1
National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
2
National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan; Research Institute for Biological Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. Electronic address: shimoji@affrc.go.jp.

Abstract

The differentiation of vaccine from non-vaccine isolates is important for disease control. Based on single nucleotide polymorphisms identified by comparison of the genomes of the Koganei 65-0.15 vaccine strain and a reference strain of Erysipelothrix rhusiopathiae, we developed a PCR assay that can differentiate the vaccine strain from field isolates.

KEYWORDS:

Erysipelothrix rhusiopathiae; Genome sequencing; SNP-based PCR; Strain-specific detection; Vaccine strain

PMID:
26151408
DOI:
10.1016/j.mimet.2015.07.001
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center