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J Biomech. 2015 Sep 18;48(12):3440-6. doi: 10.1016/j.jbiomech.2015.05.035. Epub 2015 Jun 12.

Measuring microscale strain fields in articular cartilage during rapid impact reveals thresholds for chondrocyte death and a protective role for the superficial layer.

Author information

1
School of Applied and Engineering Physics, C7 Clark Hall, Cornell University, Ithaca, NY 14853, USA. Electronic address: lrb89@cornell.edu.
2
Department of Clinical Sciences, Cornell University, Ithaca, NY, USA.
3
Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA; Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, USA.
4
Department of Physics, Cornell University, Ithaca, NY, USA.

Abstract

Articular cartilage is a heterogeneous soft tissue that dissipates and distributes loads in mammalian joints. Though robust, cartilage is susceptible to damage from loading at high rates or magnitudes. Such injurious loads have been implicated in degenerative changes, including chronic osteoarthritis (OA), which remains a leading cause of disability in developed nations. Despite decades of research, mechanisms of OA initiation after trauma remain poorly understood. Indeed, although bulk cartilage mechanics are measurable during impact, current techniques cannot access microscale mechanics at those rapid time scales. We aimed to address this knowledge gap by imaging the microscale mechanics and corresponding acute biological changes of cartilage in response to rapid loading. In this study, we utilized fast-camera and confocal microscopy to achieve roughly 85 µm spatial resolution of both the cartilage deformation during a rapid (~3 ms), localized impact and the chondrocyte death following impact. Our results showed that, at these high rates, strain and chondrocyte death were highly correlated (p<0.001) with a threshold of 8% microscale strain norm before any cell death occurred. Additionally, chondrocyte death had developed by two hours after impact, suggesting a time frame for clinical therapeutics. Moreover, when the superficial layer was removed, strain - and subsequently chondrocyte death - penetrated deeper into the samples (p<0.001), suggesting a protective role for the superficial layer of articular cartilage. Combined, these results provide insight regarding the detailed biomechanics that drive early chondrocyte damage after trauma and emphasize the importance of understanding cartilage and its mechanics on the microscale.

KEYWORDS:

Arthritis initiation; Elastography; Indentation; Injury; PTOA

PMID:
26150096
PMCID:
PMC4592793
DOI:
10.1016/j.jbiomech.2015.05.035
[Indexed for MEDLINE]
Free PMC Article

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