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Mol Microbiol. 2015 Oct;98(2):193-207. doi: 10.1111/mmi.13117. Epub 2015 Aug 3.

Bacterial microcompartments: widespread prokaryotic organelles for isolation and optimization of metabolic pathways.

Author information

1
Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
2
Molecular Biology Institute, University of California, Los Angeles, CA, USA.
3
UCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles, CA, USA.
4
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA.

Abstract

Prokaryotes use subcellular compartments for a variety of purposes. An intriguing example is a family of complex subcellular organelles known as bacterial microcompartments (MCPs). MCPs are widely distributed among bacteria and impact processes ranging from global carbon fixation to enteric pathogenesis. Overall, MCPs consist of metabolic enzymes encased within a protein shell, and their function is to optimize biochemical pathways by confining toxic or volatile metabolic intermediates. MCPs are fundamentally different from other organelles in having a complex protein shell rather than a lipid-based membrane as an outer barrier. This unusual feature raises basic questions about organelle assembly, protein targeting and metabolite transport. In this review, we discuss the three best-studied MCPs highlighting atomic-level models for shell assembly, targeting sequences that direct enzyme encapsulation, multivalent proteins that organize the lumen enzymes, the principles of metabolite movement across the shell, internal cofactor recycling, a potential system of allosteric regulation of metabolite transport and the mechanism and rationale behind the functional diversification of the proteins that form the shell. We also touch on some potential biotechnology applications of an unusual compartment designed by nature to optimize metabolic processes within a cellular context.

PMID:
26148529
PMCID:
PMC4718714
DOI:
10.1111/mmi.13117
[Indexed for MEDLINE]
Free PMC Article

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