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Plant J. 2015 Sep;83(5):831-44. doi: 10.1111/tpj.12930. Epub 2015 Jul 25.

Functional characterization of mutants affected in the carbonic anhydrase domain of the respiratory complex I in Arabidopsis thaliana.

Author information

  • 1Instituto de Investigaciones Biológicas IIB/CONICET, Universidad Nacional de Mar del Plata, cc 1245, 7600, Mar del Plata, Argentina.
  • 2Instituto de Fisiología Vegetal, Universidad Nacional de La Plata/CONICET La Plata, cc 327, 1900, La Plata, Argentina.
  • 3Plant Molecular Physiology and Biotechnology Group, Institute of Developmental and Molecular Biology of Plants, Cluster of Excellence on Plant Sciences, Heinrich Heine Universität, Universitätsstraße 1, 40225, Düsseldorf, Germany.
  • 4Institute for Plant Genetics, Leibniz Universität Hannover, Herrenhäuserstraße 2, D-30419, Hannover, Germany.

Abstract

The NADH-ubiquinone oxidoreductase complex (complex I) (EC 1.6.5.3) is the main entrance site of electrons into the respiratory chain. In a variety of eukaryotic organisms, except animals and fungi (Opisthokonta), it contains an extra domain comprising trimers of putative γ-carbonic anhydrases, named the CA domain, which has been proposed to be essential for assembly of complex I. However, its physiological role in plants is not fully understood. Here, we report that Arabidopsis mutants defective in two CA subunits show an altered photorespiratory phenotype. Mutants grown in ambient air show growth retardation compared to wild-type plants, a feature that is reversed by cultivating plants in a high-CO2 atmosphere. Moreover, under photorespiratory conditions, carbon assimilation is diminished and glycine accumulates, suggesting an imbalance with respect to photorespiration. Additionally, transcript levels of specific CA subunits are reduced in plants grown under non-photorespiratory conditions. Taken together, these results suggest that the CA domain of plant complex I contributes to sustaining efficient photosynthesis under ambient (photorespiratory) conditions.

KEYWORDS:

Arabidopsis thaliana; carbon recycling; mitochondria; photorespiration; γ-carbonic anhydrases

PMID:
26148112
DOI:
10.1111/tpj.12930
[PubMed - indexed for MEDLINE]
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