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Nat Methods. 2015 Aug;12(8):743-6. doi: 10.1038/nmeth.3466. Epub 2015 Jul 6.

Multitarget super-resolution microscopy with high-density labeling by exchangeable probes.

Author information

1
Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan.
2
Laboratory of Single-Molecule Cell Biology, Tohoku University Graduate School of Life Sciences, Sendai, Japan.
3
1] Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan. [2] Laboratory of Single-Molecule Cell Biology, Kyoto University Graduate School of Biostudies, Kyoto, Japan.

Abstract

We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment-based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the area-specific proximity between cytoskeletal components and focal adhesions within a single cell.

PMID:
26147917
DOI:
10.1038/nmeth.3466
[Indexed for MEDLINE]
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