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Cornea. 2015 Sep;34(9):1175-8. doi: 10.1097/ICO.0000000000000522.

Stamping an S on DMEK Donor Tissue to Prevent Upside-Down Grafts: Laboratory Validation and Detailed Preparation Technique Description.

Author information

1
*Cornea Service, Massachusetts Eye and Ear Infirmary, Boston, MA; †Cornea Service, Devers Eye Institute, Portland, OR; and ‡Lions VisionGift, Portland, OR.

Abstract

PURPOSE:

To report endothelial cell loss (ECL) caused by a novel S-stamp preparation technique for Descemet membrane endothelial keratoplasty (DMEK).

METHODS:

Six cadaveric human corneas were prepared for DMEK transplantation using a single standardized technique, including the application of a dry ink gentian violet S-stamp to the stromal side of Descemet membrane. Endothelial cell death was evaluated and quantified using computerized analysis of vital dye staining.

RESULTS:

ECL caused by the S-stamp was 0.6% (range 0.1%-1.0%), which comprised less than one-tenth of the total ECL caused by our preparation of the DMEK graft from the start to finish, including recovery, prestripping, S-stamping, and trephination (13.7% total ECL, range 9.9%-17.6%).

CONCLUSIONS:

Our novel S-stamp donor tissue preparation technique is intuitive to learn and holds the promise of preventing iatrogenic primary graft failure due to upside-down grafts without causing unacceptable increases in ECL.

PMID:
26147839
DOI:
10.1097/ICO.0000000000000522
[Indexed for MEDLINE]

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