(A) Expected images of the simple particle model at various beam flux (5,10,30,50, and 100 μW). Each expected images are generated by averaging 30 images over 30 sec exposure period. Intensity histograms are also shown below each expected images and presented with black-colored bars. Each histograms are logarithmically scaled and presented with grey-colored bars. (B) Simulated digital images of the simple particle model are shown for various beam flux (5,10,30,50, and 100 μW). Size of each images is 100 × 100 pixel. Orange scalebar represents 5.18 μm. Intensity histograms are also shown below each simulated images. (C) Real captured images obtained from in vitro experiment are shown for various beam flux (5,10,30,50, and 100 μW). Size of each images is 100 × 100 pixel. The maximum value of the grayscale is adjusted to improve visualization of each image. Intensity histograms are also shown below each actual images.