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PLoS One. 2015 Jul 6;10(7):e0131103. doi: 10.1371/journal.pone.0131103. eCollection 2015.

The Tinkerbell (Tink) Mutation Identifies the Dual-Specificity MAPK Phosphatase INDOLE-3-BUTYRIC ACID-RESPONSE5 (IBR5) as a Novel Regulator of Organ Size in Arabidopsis.

Author information

1
ARC Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, Royal Parade, Parkville, Victoria, 3010, Australia.
2
Institute for Biochemistry and Biology, University of Potsdam, 14476, Potsdam, Germany.
3
Sainsbury Laboratory, Cambridge University, Cambridge, CB2 1LR, United Kingdom.
4
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0192, Japan.
5
Department of Cell and Developmental Biology, John Innes Centre, Norwich, NR4 7UH, United Kingdom.

Abstract

Mitogen-activated dual-specificity MAPK phosphatases are important negative regulators in the MAPK signalling pathways responsible for many essential processes in plants. In a screen for mutants with reduced organ size we have identified a mutation in the active site of the dual-specificity MAPK phosphatase indole-3-butyric acid-response5 (IBR5) that we named tinkerbell (tink) due to its small size. Analysis of the tink mutant indicates that IBR5 acts as a novel regulator of organ size that changes the rate of growth in petals and leaves. Organ size and shape regulation by IBR5 acts independently of the KLU growth-regulatory pathway. Microarray analysis of tink/ibr5-6 mutants identified a likely role for this phosphatase in male gametophyte development. We show that IBR5 may influence the size and shape of petals through auxin and TCP growth regulatory pathways.

PMID:
26147117
PMCID:
PMC4492785
DOI:
10.1371/journal.pone.0131103
[Indexed for MEDLINE]
Free PMC Article

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