Regulation of B3GNT5 gene expression. (A) Schematic representation of B3GNT5 gene structure (A1: white boxes for untranslated regions, gray box for protein-coding regions []) and predicted CpG island (A2 []), showing 6 putative NF-κB (in dark blue) or NF-κB1 binding sites (in light blue, A3), all of which overlapping with open chromatin areas according to DNAse I hypersensitivity sites (in green, A4 []). Also displayed are the results for the NF-κB or NF-κB1 putative binding sites sequence conservation analysis across human, 5 primates and mouse: in red, conserved nucleotides; in black, nucleotides or gaps not conserved with the human sequence (Hs for Homo sapiens, Pt for Pan troglodytes, Gg for Gorilla gorilla, Pa for Pongo abelii, Cj for Callithrix jacchus and Mm for Mus musculus, A5). (B) Immunofluorescence labeling for p65 (NF-κB-pathway element) in MKN45 cell line stimulated with TNF-α in the absence or presence of NF-κB activation inhibitor IV at 20 min, 2 h or 7 h time points. Cell nuclei are stained with DAPI. Magnification 630×. (C) qRT-PCR analysis of B3GNT5 gene expression in MKN45 cell line stimulated with TNF-α in the absence or presence of NF-κB activation inhibitor IV at 20 min, 2 h or 7 h time points. The graph represents average value and SD of two independent experiments performed in triplicate. Significance was evaluated with Student’s T-test. *p < 0.05 and **p < 0.001.