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Phytomedicine. 2015 Jul 15;22(7-8):768-77. doi: 10.1016/j.phymed.2015.05.053. Epub 2015 Jun 3.

Glycyrrhizin, silymarin, and ursodeoxycholic acid regulate a common hepatoprotective pathway in HepG2 cells.

Author information

1
Department of Microbiology, China Medical University, Taichung 40402, Taiwan.
2
School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
3
Graduate Institute of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
4
Graduate Institute of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan. Electronic address: cyhsiang@mail.cmu.edu.tw.

Abstract

BACKGROUND:

Glycyrrhizin, silymarin, and ursodeoxycholic acid are widely used hepatoprotectants for the treatment of liver disorders, such as hepatitis C virus infection, primary biliary cirrhosis, and hepatocellular carcinoma.

PURPOSE:

The gene expression profiles of HepG2 cells responsive to glycyrrhizin, silymarin, and ursodeoxycholic acid were analyzed in this study.

METHODS:

HepG2 cells were treated with 25 µM hepatoprotectants for 24 h. Gene expression profiles of hepatoprotectants-treated cells were analyzed by oligonucleotide microarray in triplicates. Nuclear factor-κB (NF-κB) activities were assessed by luciferase assay.

RESULTS:

Among a total of 30,968 genes, 252 genes were commonly regulated by glycyrrhizin, silymarin, and ursodeoxycholic acid. These compounds affected the expression of genes relevant various biological pathways, such as neurotransmission, and glucose and lipid metabolism. Genes involved in hepatocarcinogenesis, apoptosis, and anti-oxidative pathways were differentially regulated by all compounds. Moreover, interaction networks showed that NF-κB might play a central role in the regulation of gene expression. Further analysis revealed that these hepatoprotectants inhibited NF-κB activities in a dose-dependent manner.

CONCLUSION:

Our data suggested that glycyrrhizin, silymarin, and ursodeoxycholic acid regulated the expression of genes relevant to apoptosis and oxidative stress in HepG2 cells. Moreover, the regulation by these hepatoprotectants might be relevant to the suppression of NF-κB activities.

KEYWORDS:

Glycyrrhizin; Hepatoprotectants; Microarray; Silymarin; Ursodeoxycholic acid

PMID:
26141764
DOI:
10.1016/j.phymed.2015.05.053
[Indexed for MEDLINE]

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