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J Biomed Opt. 2015 Jul;20(7):78002. doi: 10.1117/1.JBO.20.7.078002.

Photobiomodulation with low-level diode laser promotes osteoblast migration in an in vitro micro wound model.

Author information

1
Rizzoli Orthopaedic Institute, Laboratory of Preclinical and Surgical Studies, via di Barbiano 1/10, Bologna 40136, Italy.
2
University of Bologna, Unit of Orthodontics, Department of Biomedical and Neuromotor Sciences, via San Vitale 59, Bologna 40125, Italy.
3
Rizzoli Orthopaedic Institute, Laboratory of Preclinical and Surgical Studies, via di Barbiano 1/10, Bologna 40136, ItalycUniversity of Bologna, Department of Medical and Surgical Sciences, via Massarenti 9, Bologna 40138, Italy.
4
University of Bologna, Unit of Periodontology, Department of Biomedical and Neuromotor Sciences, via San Vitale 59, Bologna 40125, Italy.

Abstract

Laser photobiomodulation can improve bone healing, but well-defined treatment parameters are lacking. Saos-2 human osteoblast-like cells were subjected to an in vitro scratch-wound healing assay and irradiated by a 915-nm gallium-aluminum-arsenide diode laser for 0, 48, 96, and 144 s using doses of, respectively, 0, 5, 10, and 15 J/cm(2) . Wound area was measured after 4, 24, 48, and 72 h. Cell viability, DNA content, gene expression, and release of bone-related proteins were evaluated after 24, 48, and 72 h. Laser significantly improved wound healing compared with nonirradiated controls. Cells treated with laser doses of 5 and 10 J/cm(2) reached wound closure after 72 h, followed by 15 J/cm(2) after 96 h. With the cell proliferation inhibitor Mitomycin C, the doses of 10 and 15 J/cm(2) maintained an improved wound healing compared with controls. Laser increased collagen type 1 gene expression with higher doses inducing a longer-lasting effect, whereas transforming growth factor-beta 1 showed comparable or decreased levels in irradiated versus nonirradiated groups, with no effect on protein release. This study demonstrated that laser photobiomodulation at 915 nm promoted wound healing mainly through stimulation of cell migration and collagen deposition by osteoblasts.

PMID:
26140461
DOI:
10.1117/1.JBO.20.7.078002
[Indexed for MEDLINE]

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