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Oncol Lett. 2015 May;9(5):2129-2134. Epub 2015 Mar 2.

Gelsolin regulates proliferation, apoptosis, migration and invasion in human oral carcinoma cells.

Author information

1
Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical School, Nanjing University, Nanjing, Jiangsu, P.R. China.
2
Central Laboratory of Nanjing Stomatological Hospital, Nanjing, Jiangsu, P.R. China.
3
Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical School, Nanjing University, Nanjing, Jiangsu, P.R. China ; Central Laboratory of Nanjing Stomatological Hospital, Nanjing, Jiangsu, P.R. China.

Abstract

Gelsolin (GSN) is one of the most abundant actin-binding proteins, and is involved in several pathological processes, including Alzheimer's disease, cardiac injury and cancer. The aim of the present study was to assess the effect of GSN on the growth and motility of oral squamous cell carcinoma Tca8113 cells. The overexpression vector pcDNA3.1-GSN was transfected into Tca8113 cells and the stable GSN overexpression cell line was identified based on G418 antibiotic selection. The effect of GSN overexpression on the proliferation, apoptosis, migration and invasion of Tca8113 cells was examined using a cell counting kit-8 assay, flow cytometry and Transwell assays. The results revealed that GSN overexpression significantly promoted the cell proliferation and apoptosis of Tca8113 cells. In addition, Transwell assays demonstrated that the migration and invasion abilities of Tca8113 cells were enhanced by GSN overexpression. Therefore, the upregulation of GSN promotes cell growth and motility, indicating that it may perform a vital function in the progression of human oral cancers.

KEYWORDS:

apoptosis; cell motility; cell proliferation; gelsolin; oral cancer; squamous cell carcinoma

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