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Genome Med. 2015 Jun 9;7(1):52. doi: 10.1186/s13073-015-0176-9. eCollection 2015.

Phylogenetically typing bacterial strains from partial SNP genotypes observed from direct sequencing of clinical specimen metagenomic data.

Author information

1
Department of Pathogen Genomics, Translational Genomics Research Institute, Flagstaff, AZ USA ; Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ 86011 USA.
2
Department of Pathogen Genomics, Translational Genomics Research Institute, Flagstaff, AZ USA.
3
Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD USA.
4
Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ 86011 USA ; Current address: Department of Molecular, Cellular & Biomedical Sciences, University of New Hampshire, Durham, NH USA.

Abstract

We describe an approach for genotyping bacterial strains from low coverage genome datasets, including metagenomic data from complex samples. Sequence reads from unknown samples are aligned to a reference genome where the allele states of known SNPs are determined. The Whole Genome Focused Array SNP Typing (WG-FAST) pipeline can identify unknown strains with much less read data than is needed for genome assembly. To test WG-FAST, we resampled SNPs from real samples to understand the relationship between low coverage metagenomic data and accurate phylogenetic placement. WG-FAST can be downloaded from https://github.com/jasonsahl/wgfast.

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