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Angew Chem Int Ed Engl. 2015 Aug 3;54(32):9162-85. doi: 10.1002/anie.201410653. Epub 2015 Jul 1.

Facing and Overcoming Sensitivity Challenges in Biomolecular NMR Spectroscopy.

Author information

1
GE Healthcare, Broendby, Denmark; Department of Electrical Engineering, Technical University of Denmark, Danish Research Centre for Magnetic Resonance, Copenhagen University Hospital Hvidovre (Denmark).
2
U.S. National High Magnetic Field Lab, Florida State University, Tallahassee, FL 32310 (USA).
3
Institute of Physics of Biological Systems, Ecole Polytechnique Fédérale de Lausanne, Lausanne (Switzerland).
4
Department of Chemistry, University of York, Heslington, York, YO10 5DD (UK).
5
Department of Biochemistry & Molecular Biology, University of Florida, Gainesville, FL 32610 (USA).
6
Bruker Biospin GmbH, 76287 Rheinstetten (Germany).
7
MPI for Biophysical Chemistry, Göttingen (Germany).
8
Department of Chemistry and Francis Bitter Magnet Lab, MIT, Cambridge, MA 02139-4703 (USA).
9
Department of Chemistry, Texas A&M University, College Station (USA).
10
Riken Center for Life Science Technologies, Yokohama, Kanagawa (Japan).
11
CERM and Department of Chemistry, University of Florence, Sesto Fiorentino (Italy).
12
Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main (Germany).
13
Radboud University, Nijmegen (The Netherlands).
14
Chemical Physics Department, Weizmann Institute of Science, Rehovot (Israel).
15
Department of Radiology, C. J. Gorter Center for High Field MRI, Leiden University Medical Center (The Netherlands).
16
CERM and Department of Chemistry, University of Florence, Sesto Fiorentino (Italy). luchinat@cerm.unifi.it.
17
Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main (Germany). schwalbe@nmr.uni-frankfurt.de.
18
Chemical Physics Department, Weizmann Institute of Science, Rehovot (Israel). Lucio.Frydman@weizmann.ac.il.

Abstract

In the Spring of 2013, NMR spectroscopists convened at the Weizmann Institute in Israel to brainstorm on approaches to improve the sensitivity of NMR experiments, particularly when applied in biomolecular settings. This multi-author interdisciplinary Review presents a state-of-the-art description of the primary approaches that were considered. Topics discussed included the future of ultrahigh-field NMR systems, emerging NMR detection technologies, new approaches to nuclear hyperpolarization, and progress in sample preparation. All of these are orthogonal efforts, whose gains could multiply and thereby enhance the sensitivity of solid- and liquid-state experiments. While substantial advances have been made in all these areas, numerous challenges remain in the quest of endowing NMR spectroscopy with the sensitivity that has characterized forms of spectroscopies based on electrical or optical measurements. These challenges, and the ways by which scientists and engineers are striving to solve them, are also addressed.

KEYWORDS:

NMR probeheads; NMR spectroscopy; nuclear hyperpolarization; sensitivity enhancement; ultrahigh magnetic fields

PMID:
26136394
PMCID:
PMC4943876
DOI:
10.1002/anie.201410653
[Indexed for MEDLINE]
Free PMC Article

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