Format

Send to

Choose Destination
Exp Anim. 2015;64(4):435-42. doi: 10.1538/expanim.14-0086. Epub 2015 Jul 2.

A novel mice model of metabolic syndrome: the high-fat-high-fructose diet-fed ICR mice.

Author information

1
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China.

Abstract

Currently, the metabolic syndrome (MS) is occurring at growing rates worldwide, raising extensive concerns on the mechanisms and therapeutic interventions for this disorder. Herein, we described a novel method of establishing MS model in rodents. Male Institute of Cancer Research (ICR) mice were fed with high-fat-high-fructose (HFHF) diet or normal chow (NC) respectively for 12 weeks. Metabolic phenotypes were assessed by glucose tolerance test, insulin tolerance test and hyperinsulinemic-euglycemic clamp. Blood pressure was measured by a tail-cuff system. At the end of the experiment, mice were sacrificed, and blood and tissues were harvested for subsequent analysis. Serum insulin levels were measured by ELISA, and lipid profiles were determined biochemically. The HFHF diet-fed ICR mice exhibited obvious characteristics of the components of MS, including obvious obesity, severe insulin resistance, hyperinsulinemia, dislipidemia, significant hypertension and hyperuricemia. Our data suggest that HFHF diet-fed ICR mice may be a robust and efficient animal model that could well mimic the basic pathogenesis of human MS.

PMID:
26134356
PMCID:
PMC4637381
DOI:
10.1538/expanim.14-0086
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for J-STAGE, Japan Science and Technology Information Aggregator, Electronic Icon for PubMed Central
Loading ...
Support Center