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Int J Biol Macromol. 2015 Sep;80:392-9. doi: 10.1016/j.ijbiomac.2015.06.053. Epub 2015 Jun 28.

Isolation and prebiotic activity of inulin-type fructan extracted from Pfaffia glomerata (Spreng) Pedersen roots.

Author information

1
Pharmaceutical Sciences Post-Graduate Program, Department of Pharmacy, State University of Maringá, Avenida Colombo, 5790, CEP 87.020-900 Maringá, PR, Brazil.
2
Department of Microbiology and Technology, Dairy Research Institute, Ltd., Ke Dvoru 12a, 160 00 Prague, Czech Republic.
3
Department of Biochemistry and Molecular Biology, Federal University of Paraná, CP 19046, CEP 81.531-980 Curitiba, PR, Brazil.
4
Pharmaceutical Sciences Post-Graduate Program, Department of Pharmacy, State University of Maringá, Avenida Colombo, 5790, CEP 87.020-900 Maringá, PR, Brazil. Electronic address: ajboliveira@uem.br.

Abstract

Pfaffia glomerata (Amaranthaceae) is popularly known as "Brazilian ginseng." Previous studies have shown that fructose is the major carbohydrate component present in its roots. Inulin-type fructans, polymers of fructose, are the most widespread and researched prebiotics. Here, we isolated and chemically characterized inulin extracted from P. glomerata roots and investigated its potential prebiotic effect. Fructans were isolated and their structures were determined using colorimetric, chromatography, polarimetry, and spectroscopic analysis. The degree of polymerization (DP) was determined, and an in vitro prebiotic test was performed. The structure of inulin was confirmed by chromatography and spectroscopic analysis and through comparison with existing data. Representatives from the genera Lactobacillus and Bifidobacterium utilized inulin from P. glomerata, because growth was significantly stimulated, while this ability is strain specific. The results indicated that inulin extracted from P. glomerata roots represents a promising new source of inulin-type prebiotics.

KEYWORDS:

Amaranthaceae; Inulin; Pfaffia glomerata; Prebiotic

PMID:
26126944
DOI:
10.1016/j.ijbiomac.2015.06.053
[Indexed for MEDLINE]
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