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J Cereb Blood Flow Metab. 2015 Nov;35(11):1711-21. doi: 10.1038/jcbfm.2015.149. Epub 2015 Jul 1.

Multimodal imaging reveals temporal and spatial microglia and matrix metalloproteinase activity after experimental stroke.

Author information

1
European Institute for Molecular Imaging (EIMI), Westfälische Wilhelms University Münster, Münster, Germany.
2
Paris Centre de Recherche Cardiovasculaire (PARC), Paris, France.
3
Department of Clinical Radiology of the University Hospital, Westfälische Wilhelms University Münster, Münster, Germany.
4
Department of Nuclear Medicine of the University Hospital, Westfälische Wilhelms University Münster, Münster, Germany.
5
Cells-in-Motion Cluster of Excellence (EXC 1003-CiM), Westfälische Wilhelms University Münster, Münster, Germany.
6
Service Hospitalier Frédéric Joliot, Institut d'Imagerie BioMédicale, CEA, Orsay, France.
7
Institute of Biostructure and Bioimaging, CNR, Naples, Italy.
8
Institut d'Investigacions Biomèdiques de Barcelona, Consejo Superior de Investigaciones Científicas (CSIC), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
9
Institute of Physiological Chemistry and Pathobiochemistry, Westfälische Wilhelms University Münster, Münster, Germany.
10
Department of Geriatrics, Johanniter Hospital, Evangelische Kliniken, Bonn, Germany.

Abstract

Stroke is the most common cause of death and disability from neurologic disease in humans. Activation of microglia and matrix metalloproteinases (MMPs) is involved in positively and negatively affecting stroke outcome. Novel, noninvasive, multimodal imaging methods visualizing microglial and MMP alterations were employed. The spatio-temporal dynamics of these parameters were studied in relation to blood flow changes. Micro positron emission tomography (μPET) using [(18)F]BR-351 showed MMP activity within the first days after transient middle cerebral artery occlusion (tMCAo), followed by increased [(18)F]DPA-714 uptake as a marker for microglia activation with a maximum at 14 days after tMCAo. The inflammatory response was spatially located in the infarct core and in adjacent (penumbral) tissue. For the first time, multimodal imaging based on PET, single photon emission computed tomography, and magnetic resonance imaging revealed insight into the spatio-temporal distribution of critical parameters of poststroke inflammation. This allows further evaluation of novel treatment paradigms targeting the postischemic inflammation.

PMID:
26126867
PMCID:
PMC4635244
DOI:
10.1038/jcbfm.2015.149
[Indexed for MEDLINE]
Free PMC Article

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