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Genet Mol Res. 2015 Jun 11;14(2):6330-9. doi: 10.4238/2015.June.11.8.

Association of MDR1 C3435T and C1236T single nucleotide polymorphisms with male factor infertility.

Author information

1
Department of Medical Biology, School of Medicine, Ankara University, Ankara, Turkey saydos@gmail.com.
2
Department of Medical Biology, School of Medicine, Ankara University, Ankara, Turkey.
3
Vocational School of Health, Medical Laboratory Techniques, Ankara University, Ankara, Turkey.
4
Reproductive Health Research Center, School of Medicine, Ankara University, Ankara, Turkey.

Abstract

Infertility affects 1 in 6 couples and approximately 1 in 25 men. Male factor infertility is a major cause of spermatogenic anomalies, the causes of which are largely unknown. Impaired repro-ductive functions in men might result from physiological, genetic, and/or environmental factors such as xenobiotics. The multi-drug re-sistance1 (MDR1) gene encodes a P-glycoprotein which has a role in the active transport of various substrates providing protection of somatic cells from potentially toxic substances, including xenobi-otics. MDR1 is highly expressed at the luminal surface of capillary endothelial cells, and is expressed in Leydig cells, testicular mac-rophages, and Sertoli cells. We performed genotype and haplotype analyses of MDR1 in 192 infertile and 102 fertile Turkish men for the genetic markers C1236T and C3435T, using polymerase chain reaction-restriction fragment length polymorphism analysis. In the overall population, correlations were analyzed in all genotype mod-els. We found that the C3435T polymorphism TT vs CT genotypes showed statistically significant differences in their association with infertility (P = 0.045), and that the CT genotype was associated with high sperm DNA damage (P = 0.02), suggesting that the CT genotype might be a susceptibility factor for infertility. Additionally, the T-T haplotype was significantly more frequent in the control group (13.2 vs 6.5%; odds ratio = 0.459, 95%CI = 0.259-0.814, P = 0.006). This study showed that MDR1 might have a role in male infertility. Fur-ther research in large cohorts with different populations is required to clarify the role of MDR in male fertility.

PMID:
26125837
DOI:
10.4238/2015.June.11.8
[Indexed for MEDLINE]
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