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Nat Methods. 2015 Jul;12(7):605-8. doi: 10.1038/nmeth.3450.

Mass spectrometrists should search only for peptides they care about.

Author information

1
Department of Genome Sciences and the Department of Computer Science and Engineering, University of Washington, Seattle, Washington, USA.

Abstract

Analysis pipelines that assign peptides to shotgun proteomics mass spectra often discard identified spectra deemed irrelevant to the scientific hypothesis being tested. To improve statistical power, I propose that researchers remove irrelevant peptides from the database prior to searching rather than assigning these peptides to spectra and then discarding the matches.

PMID:
26125591
PMCID:
PMC4711994
DOI:
10.1038/nmeth.3450
[Indexed for MEDLINE]
Free PMC Article

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