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Anticancer Res. 2015 Jul;35(7):3943-51.

Phthalocyanine-mediated Photodynamic Treatment of Tumoural and Non-tumoural cell lines.

Author information

1
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic barbora.manisova@upol.cz.
2
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
3
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.

Abstract

This study deals with the use of cationic far-red absorbing photosensitizers (λ(max) ~740 nm) from the group of the phthalocyanines, in photodynamic therapy. The photosensitizers differed in their central atom, bearing either hydrogen, zinc or magnesium. These photosensitizers were tested in vitro on the tumour cell line HeLa (cervical cancer) and non-tumour cell line NIH3T3 (mouse fibroblast). The following tests were performed: measurement of reactive oxygen species production, viability testing, Comet assay and cell type detection (apoptotic, necrotic and living cells). The best results were achieved with zinc derivative at relatively low half-maximum inhibitory concentration (0.04 μM) and a total radiation dose of 15 J cm(-2).

KEYWORDS:

Photosensitizer; Phthalocyanine; cellular damage; oxidative stress; photodynamic treatment; phototoxicity; tumour cells

PMID:
26124341
[Indexed for MEDLINE]

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