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Anticancer Res. 2015 Jul;35(7):3867-73.

Curcumin Triggers DNA Damage and Inhibits Expression of DNA Repair Proteins in Human Lung Cancer Cells.

Author information

1
Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei, Taiwan, R.O.C. Department of Medical Imaging and Radiology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan, R.O.C.
2
Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei, Taiwan, R.O.C.
3
School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C.
4
Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
5
Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei, Taiwan, R.O.C. Department of Radiation Oncology, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C.
6
Department of Radiation Oncology, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C. Department of Radiological Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. john740604@yahoo.com.tw.

Abstract

The study goal was to evaluate the effects of curcumin on DNA damage and expression of DNA-repair proteins in human lung cancer. Thus, NCI-H460 cells were used to study the effects of curcumin on DNA damage and repair in vitro. We investigated curcumin induces DNA damage by comet the assay and 4',6-diamidino-2-phenylindole (DAPI) staining. The DNA damage/repair-related protein levels were examined and monitored by western blotting and confocal microscopy. Curcumin significantly increased the length of comet tails and DNA condensation in NCI-H460 cells. Curcumin reduced expression of DNA-repair proteins such as 14-3-3 protein sigma (14-3-3σ), O6-methylguanine-DNA methyltransferase (MGMT), breast cancer susceptibility gene 1 (BRCA1), and mediator of DNA damage checkpoint 1 (MDC1). Curcumin also increased phosphorylation of p53 and Histone H2A.X (S140) in the nuclei of NCI-H460 cells. Taken together, our findings indicated that curcumin triggered DNA damage and inhibited expression of DNA-repair-associated proteins in NCI-H460 cells.

KEYWORDS:

Curcumin; DNA damage; DNA repair; human lung cancer

PMID:
26124332
[Indexed for MEDLINE]

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