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Otolaryngol Head Neck Surg. 2015 Dec;153(6):1024-30. doi: 10.1177/0194599815591810. Epub 2015 Jun 29.

Expression of Apoptotic vs Antiapoptotic Proteins in Middle Ear Cholesteatoma.

Author information

1
Departments of Otolaryngology-Head and Neck Surgery, School of Medicine, Hanyang University, Seoul, Korea.
2
Departments of Otolaryngology-Head and Neck Surgery, School of Medicine, Hanyang University, Seoul, Korea Shleemd@hanyang.ac.kr.
3
Department of Otorhinolaryngology, Konkuk University School of Medicine, Chungju, Korea.
4
Departments of Pathology, School of Medicine, Hanyang University, Seoul, Korea.

Abstract

OBJECTIVES:

To explore the role of antiapoptotic and apoptotic processes in the development of cholesteatoma by investigating the expression of an antiapoptotic (c-FLIP) and apoptotic (p53) protein relative to the expression of a proliferation marker (Ki-67).

STUDY DESIGN:

Basic science study.

SETTING:

Tertiary referral center.

SUBJECTS AND METHODS:

An immunohistochemical investigation was performed on 35 cholesteatoma specimens (21 acquired, 14 congenital) and 10 normal retroauricular skins to evaluate the expression of c-FLIP, p53, and Ki-67. The expression rate of each marker was measured to assess the difference between retroauricular skin and cholesteatoma, as well as between congenital and acquired cholesteatoma.

RESULTS:

c-FLIP expression was significantly higher in the cholesteatoma specimens than in retroauricular skin (P < .05), while the expression of p53 did not significantly differ between the two. Ki-67 expression in cholesteatoma was significantly higher than in retroauricular skin (P < .001). The c-FLIP expression rate was positively correlated with that of Ki-67 (r = 0.47, P = .001), and there was no significant correlation between the expression level of p53 and that of Ki-67 (r = 0.152, P = .319). In addition, no differences in c-FLIP, p53, and Ki-67 expression rates were evident between congenital and acquired cholesteatoma.

CONCLUSIONS:

The upregulation of c-FLIP together with unchanged p53 suggests an altered equilibrium between apoptosis and antiapoptosis, favoring antiapoptosis, and may play a role in the pathogenesis of cholesteatoma.

KEYWORDS:

Ki-67; apoptosis; c-FLIP; cholesteatoma; p53

PMID:
26124267
DOI:
10.1177/0194599815591810
[Indexed for MEDLINE]

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