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Proc Natl Acad Sci U S A. 2015 Jul 14;112(28):8644-9. doi: 10.1073/pnas.1504259112. Epub 2015 Jun 29.

Phosphatidylinositol-4-phosphate-dependent membrane traffic is critical for fungal filamentous growth.

Author information

1
Université de Nice-Sophia Antipolis, Institute of Biology Valrose, Parc Valrose, 06108 Nice Cedex 2, France; CNRS Institute of Biology Valrose, UMR7277, Parc Valrose, 06108 Nice Cedex 2, France; INSERM Institute of Biology Valrose, UMR1091, Parc Valrose, 06108 Nice Cedex 2, France;
2
CNRS, Université de Nice-Sophia Antipolis, Laboratoire de Physique de la Matière Condensée-UMR 7336, Avenue J. Vallot, 06108 Nice Cedex 2, France.
3
Université de Nice-Sophia Antipolis, Institute of Biology Valrose, Parc Valrose, 06108 Nice Cedex 2, France; CNRS Institute of Biology Valrose, UMR7277, Parc Valrose, 06108 Nice Cedex 2, France; INSERM Institute of Biology Valrose, UMR1091, Parc Valrose, 06108 Nice Cedex 2, France; arkowitz@unice.fr.

Abstract

The phospholipid phosphatidylinositol-4-phosphate [PI(4)P], generated at the Golgi and plasma membrane, has been implicated in many processes, including membrane traffic, yet its role in cell morphology changes, such as the budding to filamentous growth transition, is unknown. We show that Golgi PI(4)P is required for such a transition in the human pathogenic fungus Candida albicans. Quantitative analyses of membrane traffic revealed that PI(4)P is required for late Golgi and secretory vesicle dynamics and targeting and, as a result, is important for the distribution of a multidrug transporter and hence sensitivity to antifungal drugs. We also observed that plasma membrane PI(4)P, which we show is functionally distinct from Golgi PI(4)P, forms a steep gradient concomitant with filamentous growth, despite uniform plasma membrane PI-4-kinase distribution. Mathematical modeling indicates that local PI(4)P generation and hydrolysis by phosphatases are crucial for this gradient. We conclude that PI(4)P-regulated membrane dynamics are critical for morphology changes.

KEYWORDS:

filamentous growth; lipid distribution; membrane traffic; morphogenesis; polarity

PMID:
26124136
PMCID:
PMC4507248
DOI:
10.1073/pnas.1504259112
[Indexed for MEDLINE]
Free PMC Article

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