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Infect Immun. 2015 Sep;83(9):3601-11. doi: 10.1128/IAI.00217-15. Epub 2015 Jun 29.

Role of CD4+ Foxp3+ Regulatory T Cells in Protection Induced by a Live Attenuated, Replicating Type I Vaccine Strain of Toxoplasma gondii.

Author information

1
Université de Tours, UMR1282 Infectiologie et Santé Publique, Tours, France, and INRA, UMR1282 Infectiologie et Santé Publique, Nouzilly, France.
2
Université de Tours, UMR1282 Infectiologie et Santé Publique, Tours, France, and INRA, UMR1282 Infectiologie et Santé Publique, Nouzilly, France nathalie.moire@univ-tours.fr.

Abstract

Vaccination with the live attenuated Toxoplasma gondii Mic1.3KO strain induced long-lasting immunity against challenge with Toxoplasma gondii type I and type II strains. The involvement of regulatory T cells (Tregs) in the protection mechanism was investigated. Intraperitoneal injection of Mic1.3KO induced a weak and transient influx of CD4(+) Foxp3(+) T regulatory cells followed by recruitment/expansion of CD4(+) Foxp3(-) CD25(+) effector cells and control of the parasite at the site of infection. The local and systemic cytokine responses associated with this recruitment of Tregs were of the TH1/Treg-like type. In contrast, injection of RH, the wild-type strain from which the vaccinal strain is derived, induced a low CD4(+) Foxp3(+) cell influx and uncontrolled multiplication of the parasites at this local site, followed by death of the mice. The associated local and systemic cytokine responses were of the TH1/TH17-like type. In addition, in vivo Treg induction in RH-infected mice with interleukin-2 (IL-2)/anti-IL-2 complexes induced control of the parasite and a TH1/Treg cytokine response similar to the response after Mic1.3KO vaccination. These results suggest that Tregs may contribute to the protective response after vaccination with Mic1.3KO.

PMID:
26123802
PMCID:
PMC4534668
DOI:
10.1128/IAI.00217-15
[Indexed for MEDLINE]
Free PMC Article

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