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BMC Res Notes. 2015 Jun 30;8:279. doi: 10.1186/s13104-015-1228-3.

Antioxidant and phytochemical analysis of Ranunculus arvensis L. extracts.

Author information

1
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan. zeshan34@yahoo.com.
2
Institute of Biomedical Sciences, School of Life Science, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, People's Republic of China. amjad_486@yahoo.com.
3
Department of Biotechnology, Abdul Wali Khan University, Mardan, 23200, Pakistan. ahdayazb5@awkum.edu.pk.
4
Department of Biological Sciences, Gomal University, Dera Ismail Khan, 29050, Pakistan. asmasaeed78@gmail.com.
5
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan. samalikqau@yahoo.com.

Abstract

BACKGROUND:

Ranunculus arvensis L. (R. arvensis) has long been used to treat a variety of medical conditions such as arthritis, asthma, hay fever, rheumatism, psoriasis, gut diseases and rheumatic pain. Here, we screened R. arvensis for antioxidant activity, phytochemical and high performance liquid chromatography (HPLC) analyses.

METHODS:

The chloroform, chloroform:methanol, methanol, methanol:acetone, acetone, methanol:water and water extracts of R. arvensis were examined for DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical scavenging assay, hydrogen peroxide scavenging assay, phosphomolybdenum assay, reducing power assay, flavonoid content, phenolic content and high performance liquid chromatography analysis.

RESULTS:

Significant antioxidant activity was displayed by methanol extract (IC 50 34.71 ± 0.02) in DPPH free radical scavenging assay. Total flavonoids and phenolics ranged 0.96-6.0 mg/g of extract calculated as rutin equivalent and 0.48-1.43 mg/g of extract calculated as gallic acid equivalent respectively. Significant value of rutin and caffeic acid was observed via high performance liquid chromatography.

CONCLUSIONS:

These results showed that extracts of R. arvensis exhibited significant antioxidant activities. Moreover, R. arvensis is a rich source of rutin, flavonoids and phenolics.

PMID:
26123646
PMCID:
PMC4485861
DOI:
10.1186/s13104-015-1228-3
[Indexed for MEDLINE]
Free PMC Article

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