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Chem Commun (Camb). 2015 Aug 4;51(60):12107-10. doi: 10.1039/c5cc03769g.

Sortase A-mediated multi-functionalization of protein nanoparticles.

Author information

1
Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA. wilfred@udel.edu.

Abstract

We report here a new strategy to enable fast, covalent, and site-directed functionalization of protein nanoparticles using Sortase A-mediated ligation using functional proteins ranging from monomeric to large tetrameric structures. Easy purification of the modified E2 nanoparticles is achieved by functionalization with a thermo-responsive elastin-like-peptide. The resulting protein nanoparticles remained intact and active even after repeated phase transitions, suggesting their use in biocatalysis, biosensing, and imaging applications.

PMID:
26120946
DOI:
10.1039/c5cc03769g
[Indexed for MEDLINE]

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