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Future Microbiol. 2015;10(7):1125-31. doi: 10.2217/fmb.15.38. Epub 2015 Jun 29.

Increase of widespread A, B1 and D Escherichia coli clones producing a high diversity of CTX-M-types in a Portuguese hospital.

Author information

1
UCIBIO/REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.
2
CEBIMED/FP-ENAS, Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Porto, Portugal.
3
Centro Hospitalar do Porto - Hospital de Santo António, Porto, Portugal.

Abstract

AIM:

To characterize temporal shifts in extended-spectrum β-lactamases (ESBLs) and clones of clinical Escherichia coli isolates.

MATERIALS & METHODS:

All ESBL-producing E. coli isolates from a Portuguese hospital (n = 112; June 2006-June 2007 and January-December 2010) were characterized by identification of phylogenetic groups, ESBL-types and virulence genes, XbaI-PFGE and MLST.

RESULTS:

We observed a substantial increase in widespread E. coli clones from phylogroups A, B1 and D (e.g., ST10, ST23, ST117, ST155, ST648) producing mainly CTX-M-1, -14, -32 or SHV-12, along with a decrease in the proportion of the predominant CTX-M-15-producing B2-ST131 clone.

CONCLUSION:

The amplification of diverse CTX-M-producing A, B1 and D clonal complexes, which have been long identified in Portuguese nonclinical settings, unveils a role for these reservoirs in the landscape of ESBL-producing E. coli in the clinical setting.

KEYWORDS:

ESBL; MLST; nonclinical reservoirs; phylogenetic group; widespread clonal complexes

PMID:
26119348
DOI:
10.2217/fmb.15.38
[Indexed for MEDLINE]

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