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Cell Host Microbe. 2015 Jul 8;18(1):109-21. doi: 10.1016/j.chom.2015.06.004. Epub 2015 Jun 25.

Global Mapping of the Inc-Human Interactome Reveals that Retromer Restricts Chlamydia Infection.

Author information

1
Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
2
QB3, California Institute for Quantitative Biosciences, San Francisco, CA 94148, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
3
Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA.
4
Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA.
5
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Gladstone Institutes, San Francisco, CA 94158, USA.
6
Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
7
Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, CT 06510, USA.
8
QB3, California Institute for Quantitative Biosciences, San Francisco, CA 94148, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Gladstone Institutes, San Francisco, CA 94158, USA. Electronic address: nevan.krogan@ucsf.edu.
9
Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: jengel@medicine.ucsf.edu.

Abstract

Chlamydia trachomatis is a leading cause of genital and ocular infections for which no vaccine exists. Upon entry into host cells, C. trachomatis resides within a membrane-bound compartment—the inclusion—and secretes inclusion membrane proteins (Incs) that are thought to modulate the host-bacterium interface. To expand our understanding of Inc function(s), we subjected putative C. trachomatis Incs to affinity purification-mass spectroscopy (AP-MS). We identified Inc-human interactions for 38/58 Incs with enrichment in host processes consistent with Chlamydia's intracellular life cycle. There is significant overlap between Inc targets and viral proteins, suggesting common pathogenic mechanisms among obligate intracellular microbes. IncE binds to sorting nexins (SNXs) 5/6, components of the retromer, which relocalizes SNX5/6 to the inclusion membrane and augments inclusion membrane tubulation. Depletion of retromer components enhances progeny production, revealing that retromer restricts Chlamydia infection. This study demonstrates the value of proteomics in unveiling host-pathogen interactions in genetically challenging microbes.

PMID:
26118995
PMCID:
PMC4540348
DOI:
10.1016/j.chom.2015.06.004
[Indexed for MEDLINE]
Free PMC Article

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