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Arch Biochem Biophys. 2015 Sep 1;581:49-53. doi: 10.1016/j.abb.2015.06.017. Epub 2015 Jun 24.

Validation methods for low-resolution fitting of atomic structures to electron microscopy data.

Author information

1
Bioinformatics and Structural Biology Program, Sanford-Burnham Medical Research Institute, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA.
2
Bioinformatics and Structural Biology Program, Sanford-Burnham Medical Research Institute, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA. Electronic address: niels@sbmri.org.

Abstract

Fitting of atomic-resolution structures into reconstructions from electron cryo-microscopy is routinely used to understand the structure and function of macromolecular machines. Despite the fact that a plethora of fitting methods has been developed over recent years, standard protocols for quality assessment and validation of these fits have not been established. Here, we present the general concepts underlying current validation ideas as they relate to fitting of atomic-resolution models into electron cryo-microscopy reconstructions, with an emphasis on reconstructions with resolutions below the sub-nanometer range.

KEYWORDS:

Electron microscopy; Fitting; Validation

PMID:
26116787
PMCID:
PMC4543555
DOI:
10.1016/j.abb.2015.06.017
[Indexed for MEDLINE]
Free PMC Article

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