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Ann Clin Lab Sci. 2015 Spring;45(3):301-7.

Clot Strength as Measured by Thrombelastography Correlates with Platelet Reactivity in Stroke Patients.

Author information

1
Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center at Houston, Houston, TX, USA.
2
Department of Neurology, The University of Texas Health Science Center at Houston, Houston, TX, USA.
3
Department of Neurology, The University of Texas Health Science Center at Houston, Houston, TX, USA james.c.grotta@uth.tmc.edu.

Abstract

OBJECTIVE:

Platelet reactivity may be important in the management of patients with stroke. However, degree of platelet reactivity has not been correlated with Thrombelastography (TEG(®)) parameters in stroke. We sought to detect a correlation between TEG(®) values and clot platelet reactivity in ex vivo clots of stroke patients.

METHODS:

We collected venous blood from 40 patients with stroke. TEG(®) measurements were carried out and residual clots were fixed in 10% formalin immediately following completion of TEG(®). The formalin specimens were embedded in paraffin blocks, cut at 4 micrometers, and stained with CD 61 (immunohistochemical stain used to detect platelets) with appropriate controls. Under light microscopy, three pathologists blinded to TEG(®) results independently graded CD61 intensity (how aggregated/intense the CD61 stained) into a low and high group, as a proposed measurement representing the platelet reactivity of the clot. We compared pre-tPA-TEG(®) values among groups with different CD 61 intensities.

RESULTS:

After adjusting for confounding factors, we found statistically significant correlation between CD61 staining and several TEG(®) parameters (Delta and CD61 staining intensity (p=0.047); Angle and CD61 staining intensity grade (p=0.04); and G and CD61 staining intensity grade (p=0.04)).

CONCLUSIONS:

Clot strength on TEG(®) as measured by Delta, Angle, and G correlates with a clot with greater platelet reactivity.

PMID:
26116594
[Indexed for MEDLINE]
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