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Sci Rep. 2015 Jun 26;5:11598. doi: 10.1038/srep11598.

Quantitative analysis of the human T cell palmitome.

Author information

1
Freie Universität Berlin, Institut für Chemie und Biochemie, Thielallee 63, 14195 Berlin, Germany.
2
1] Freie Universität Berlin, Institut für Chemie und Biochemie, Thielallee 63, 14195 Berlin, Germany [2] Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
3
Otto-von-Guericke University, Institute of Molecular and Clinical Immunology, Leipziger Strasse 44, 39120 Magdeburg, Germany.
4
Heidelberg University Biochemistry Center (BZH)Im Neuenheimer Feld 328 69120 Heidelberg.
5
Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.

Abstract

Palmitoylation is a reversible post-translational modification used to inducibly compartmentalize proteins in cellular membranes, affecting the function of receptors and intracellular signaling proteins. The identification of protein "palmitomes" in several cell lines raises the question to what extent this modification is conserved in primary cells. Here we use primary T cells with acyl-biotin exchange and quantitative mass spectrometry to identify a pool of proteins previously unreported as palmitoylated in vivo.

PMID:
26111759
PMCID:
PMC4650600
DOI:
10.1038/srep11598
[Indexed for MEDLINE]
Free PMC Article

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