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Pharmacognosy Res. 2015 Jun;7(Suppl 1):S34-8. doi: 10.4103/0974-8490.150522.

Hematological effects of repeated graded doses of the methanol extract of Paullinia pinnata (Linn.) leaves in Wistar albino rats.

Author information

1
Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
2
Animal Physiology Laboratory, Department of Animal Science, University of Ibadan, Ibadan, Oyo State, Nigeria.

Abstract

BACKGROUND:

Paullinia pinnata is a medicinal plant used for the treatment of various diseases, including anemia in West Africa.

AIM:

This study was carried out to investigate the effect of increasing doses of the methanolic leaves extract of P. pinnata on hematological parameters in rats.

MATERIALS AND METHODS:

Thirty-six male Wistar albino rats were grouped into six groups of six animals each. Five doses; 50,100, 200, 400 and 800 mg/kg body weight of the P. pinnata extract were administered separately to five groups. The sixth group served as a control and received only the vehicle (70% physiological saline: 30% Tween 80 [v/v]). Administration was done orally daily for 28 days at 24 h interval. On day 29, the animals were made inactive, blood was then collected from the heart and various hematological parameters were evaluated.

STATISTICAL ANALYSIS:

Analysis of variance was employed.

RESULTS:

The packed cell volume and red blood cell count increased significantly (P < 0.05) in the treatment groups except at 200 mg/kg dose. The hemoglobin concentration increased in all the treatment groups. The values for the neutrophils at 50, 100, 200 and 800 mg/kg doses were higher than that of the control. The white blood cell count increased significantly (P < 0.05) at 50 and 400 mg/kg doses compared to the control and exceeded the normal physiological range.

CONCLUSION:

The maximum tolerable dose is 200 mg/kg body weight of the methanolic leaves extract of P. pinnata and the extract has anti-anemic property with the ability to increase neutrophils count.

KEYWORDS:

Erythropoiesis; Paullinia pinnata; hematological parameters; neutrophils

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