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J Biol Chem. 2015 Aug 14;290(33):20590-600. doi: 10.1074/jbc.M115.654780. Epub 2015 Jun 24.

The Tubular Sheaths Encasing Methanosaeta thermophila Filaments Are Functional Amyloids.

Author information

1
From the Center for Microbial Communities, Department of Chemistry and Biosciences, Aalborg University, 9220 Aalborg, Denmark.
2
the Department of Biosciences.
3
the Interdisciplinary Nanoscience Center (iNANO) and Center for Insoluble Protein Structures (inSPIN), the Department of Molecular Biology and Genetics, and.
4
the Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
5
the Department of Molecular Biology and Genetics, and.
6
From the Center for Microbial Communities, Department of Chemistry and Biosciences, Aalborg University, 9220 Aalborg, Denmark, phn@bio.aau.dk.

Abstract

Archaea are renowned for their ability to thrive in extreme environments, although they can be found in virtually all habitats. Their adaptive success is linked to their unique cell envelopes that are extremely resistant to chemical and thermal denaturation and that resist proteolysis by common proteases. Here we employ amyloid-specific conformation antibodies and biophysical techniques to show that the extracellular cell wall sheaths encasing the methanogenic archaea Methanosaeta thermophila PT are functional amyloids. Depolymerization of sheaths and subsequent MS/MS analyses revealed that the sheaths are composed of a single major sheath protein (MspA). The amyloidogenic nature of MspA was confirmed by in vitro amyloid formation of recombinant MspA under a wide range of environmental conditions. This is the first report of a functional amyloid from the archaeal domain of life. The amyloid nature explains the extreme resistance of the sheath, the elastic properties that allow diffusible substrates to penetrate through expandable hoop boundaries, and how the sheaths are able to split and elongate outside the cell. The archaeal sheath amyloids do not share homology with any of the currently known functional amyloids and clearly represent a new function of the amyloid protein fold.

KEYWORDS:

amyloid; archaea; cell wall; functional amyloid; methanogen; protein folding; sheath; structure-function

PMID:
26109065
PMCID:
PMC4536462
DOI:
10.1074/jbc.M115.654780
[Indexed for MEDLINE]
Free PMC Article

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