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BMC Genomics. 2015 Jun 25;16:475. doi: 10.1186/s12864-015-1686-y.

The human cardiac and skeletal muscle proteomes defined by transcriptomics and antibody-based profiling.

Author information

1
Science for Life Laboratory, Dept of Immunology Genetics and Pathology, Uppsala University, SE-751 85, Uppsala, Sweden. cecilia.lindskog@igp.uu.se.
2
Science for Life Laboratory, Dept of Immunology Genetics and Pathology, Uppsala University, SE-751 85, Uppsala, Sweden. jerker.linne@igp.uu.se.
3
Science for Life Laboratory, KTH - Royal Institute of Technology, AlbaNova University Center, SE-171 21, Stockholm, Sweden. linn.fagerberg@scilifelab.se.
4
Science for Life Laboratory, KTH - Royal Institute of Technology, AlbaNova University Center, SE-171 21, Stockholm, Sweden. bjorn.hallstrom@scilifelab.se.
5
Department of Physiology and Pharmacology, Karolinska Institutet, SE-171 77, Stockholm, Sweden. carl.j.sundberg@ki.se.
6
Department of Physiology and Pharmacology, Karolinska Institutet, SE-171 77, Stockholm, Sweden. Malene.Lindholm@ki.se.
7
Science for Life Laboratory, Dept of Biochemistry and Biophysics, Stockholm University, Box 1031, SE-17121, Solna, Sweden. mikael.huss@scilifelab.se.
8
Science for Life Laboratory, Dept of Immunology Genetics and Pathology, Uppsala University, SE-751 85, Uppsala, Sweden. caroline.kampf@igp.uu.se.
9
NHLBI Proteomics Center at UCLA, Departments of Physiology and Medicine, Division of Cardiology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA. cjh9595@ucla.edu.
10
NHLBI Proteomics Center at UCLA, Departments of Physiology and Medicine, Division of Cardiology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA. dliemmd@gmail.com.
11
NHLBI Proteomics Center at UCLA, Departments of Physiology and Medicine, Division of Cardiology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA. pping@mednet.ucla.edu.
12
Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 58, Gothenburg, Sweden. leif.varemo@chalmers.se.
13
Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 58, Gothenburg, Sweden. adilm@chalmers.se.
14
Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 58, Gothenburg, Sweden. nielsenj@chalmers.se.
15
Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85, Uppsala, Sweden. erik.larsson@igp.uu.se.
16
Science for Life Laboratory, Dept of Immunology Genetics and Pathology, Uppsala University, SE-751 85, Uppsala, Sweden. fredrik.ponten@igp.uu.se.
17
Science for Life Laboratory, KTH - Royal Institute of Technology, AlbaNova University Center, SE-171 21, Stockholm, Sweden. mathias.uhlen@scilifelab.se.
18
NHLBI Proteomics Center at UCLA, Departments of Physiology and Medicine, Division of Cardiology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA. mathias.uhlen@scilifelab.se.

Abstract

BACKGROUND:

To understand cardiac and skeletal muscle function, it is important to define and explore their molecular constituents and also to identify similarities and differences in the gene expression in these two different striated muscle tissues. Here, we have investigated the genes and proteins with elevated expression in cardiac and skeletal muscle in relation to all other major human tissues and organs using a global transcriptomics analysis complemented with antibody-based profiling to localize the corresponding proteins on a single cell level.

RESULTS:

Our study identified a comprehensive list of genes expressed in cardiac and skeletal muscle. The genes with elevated expression were further stratified according to their global expression pattern across the human body as well as their precise localization in the muscle tissues. The functions of the proteins encoded by the elevated genes are well in line with the physiological functions of cardiac and skeletal muscle, such as contraction, ion transport, regulation of membrane potential and actomyosin structure organization. A large fraction of the transcripts in both cardiac and skeletal muscle correspond to mitochondrial proteins involved in energy metabolism, which demonstrates the extreme specialization of these muscle tissues to provide energy for contraction.

CONCLUSIONS:

Our results provide a comprehensive list of genes and proteins elevated in striated muscles. A number of proteins not previously characterized in cardiac and skeletal muscle were identified and localized to specific cellular subcompartments. These proteins represent an interesting starting point for further functional analysis of their role in muscle biology and disease.

PMID:
26109061
PMCID:
PMC4479346
DOI:
10.1186/s12864-015-1686-y
[Indexed for MEDLINE]
Free PMC Article

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