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Am J Physiol Regul Integr Comp Physiol. 2015 Aug 15;309(4):R345-57. doi: 10.1152/ajpregu.00225.2015. Epub 2015 Jun 24.

A high-throughput assay for quantifying appetite and digestive dynamics.

Author information

1
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts; and josuajordi@fas.harvard.edu.
2
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts; and Committee for Higher Degrees in Biophysics, Harvard University, Cambridge, Massachusetts.
3
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts; and.

Abstract

Food intake and digestion are vital functions, and their dysregulation is fundamental for many human diseases. Current methods do not support their dynamic quantification on large scales in unrestrained vertebrates. Here, we combine an infrared macroscope with fluorescently labeled food to quantify feeding behavior and intestinal nutrient metabolism with high temporal resolution, sensitivity, and throughput in naturally behaving zebrafish larvae. Using this method and rate-based modeling, we demonstrate that zebrafish larvae match nutrient intake to their bodily demand and that larvae adjust their digestion rate, according to the ingested meal size. Such adaptive feedback mechanisms make this model system amenable to identify potential chemical modulators. As proof of concept, we demonstrate that nicotine, l-lysine, ghrelin, and insulin have analogous impact on food intake as in mammals. Consequently, the method presented here will promote large-scale translational research of food intake and digestive function in a naturally behaving vertebrate.

KEYWORDS:

DiR' dye; appetite; hunger; satiation; satiety

PMID:
26108871
PMCID:
PMC4538228
DOI:
10.1152/ajpregu.00225.2015
[Indexed for MEDLINE]
Free PMC Article

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