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PLoS One. 2015 Jun 24;10(6):e0128902. doi: 10.1371/journal.pone.0128902. eCollection 2015.

Control of Pierce's Disease by Phage.

Author information

1
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, United States of America; Center for Phage Technology, Texas A&M University, College Station, Texas, United States of America.
2
Center for Phage Technology, Texas A&M University, College Station, Texas, United States of America; Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, United States of America.

Abstract

Pierce's Disease (PD) of grapevines, caused by Xylella fastidiosa subsp. fastidiosa (Xf), is a limiting factor in the cultivation of grapevines in the US. There are presently no effective control methods to prevent or treat PD. The therapeutic and prophylactic efficacy of a phage cocktail composed of four virulent (lytic) phages was evaluated for control of PD. Xf levels in grapevines were significantly reduced in therapeutically or prophylactically treated grapevines. PD symptoms ceased to progress one week post-therapeutic treatment and symptoms were not observed in prophylactically treated grapevines. Cocktail phage levels increased in grapevines in the presence of the host. No in planta phage-resistant Xf isolates were obtained. Moreover, Xf mutants selected for phage resistance in vitro did not cause PD symptoms. Our results indicate that phages have great potential for biocontrol of PD and other economically important diseases caused by Xylella.

PMID:
26107261
PMCID:
PMC4479439
DOI:
10.1371/journal.pone.0128902
[Indexed for MEDLINE]
Free PMC Article

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