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Microbiol Spectr. 2015 Feb;3(1):AID-0027-2014. doi: 10.1128/microbiolspec.AID-0027-2014.

Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies.

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Vanderbilt Vaccine Center and Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232.
Vanderbilt Vaccine Center and Departments of Pediatrics and Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232.


The human hybridoma technique offers an important approach for isolation of human monoclonal antibodies. A diversity of approaches can be used with varying success. Recent technical advances in expanding the starting number of human antigen-specific B cells, improving fusion efficiency, and isolating new myeloma partners and new cell cloning methods have enabled the development of protocols that make the isolation of human monoclonal antibodies from blood samples feasible. Undoubtedly, additional innovations that could improve efficiency are possible.

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