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Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2835-7. doi: 10.3109/19401736.2015.1053117. Epub 2015 Jun 24.

Two complete chloroplast genome sequences of Cannabis sativa varieties.

Author information

1
a Department of Forensic Science Investigation , Supreme Prosecutors' Office , Seoul , South Korea and.
2
b Department of Biological Sciences , Inha University , Incheon , South Korea.

Abstract

In this study, we determined the complete chloroplast (cp) genomes from two varieties of Cannabis sativa. The genome sizes were 153,848 bp (the Korean non-drug variety, Cheungsam) and 153,854 bp (the African variety, Yoruba Nigeria). The genome structures were identical with 131 individual genes [86 protein-coding genes (PCGs), eight rRNA, and 37 tRNA genes]. Further, except for the presence of an intron in the rps3 genes of two C. sativa varieties, the cp genomes of C. sativa had conservative features similar to that of all known species in the order Rosales. To verify the position of C. sativa within the order Rosales, we conducted phylogenetic analysis by using concatenated sequences of all PCGs from 17 complete cp genomes. The resulting tree strongly supported monophyly of Rosales. Further, the family Cannabaceae, represented by C. sativa, showed close relationship with the family Moraceae. The phylogenetic relationship outlined in our study is well congruent with those previously shown for the order Rosales.

KEYWORDS:

Cannabis sativa; Cheungsam; Yoruba Nigeria; chloroplast genome; narcotic plant

PMID:
26104156
DOI:
10.3109/19401736.2015.1053117
[Indexed for MEDLINE]

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